Investigate the effect of temperature on the activity of catalase Essay - Paper Example Investigate the effect of temperature on the activity of catalase Essay Introduction:
Is my conclusion valid? The more enzyme present, the more chance there is of a successful collision occurring between the substrate and the enzyme, in order to form an enzyme-substrate complex. The hydrogen peroxide will react with the prosthetic iron group in the Catalase [specifically amino acids His 74 and Asn ].
This breaks the double bond between the hydrogen heterolytically. The oxygen formed will react with the enzyme active site and displace the water that has formed there from the above reaction.
This cycle will continue until there is no Hydrogen Peroxide left. By altering its shape, the enzyme induces pressure onto the substrate, therefore lowering its activation energy.
The lock and key theory however is based on the principle of the enzymes specific nature. According to this theory, the substrate fits perfectly into the enzyme active site.
The Primary structure of Catalase is the sequence of amino acids that make up the protein [Catalase has amino acids, as well as one haem group]. The Secondary structure is the manipulation of these sequences with hydrogen bonds.
The Tertiary structure consists of other bonding types- ionic, hydrophobic, disulphide and further hydrogen bonds.
The quaternary structure of Catalase includes the prosthetic haem group. If any of these structures are altered, the protein cannot act as a site of reaction. What does it do? Catalase is an enzyme. It is a naturally occurring globular protein found residing in cell 'sacs' called Perisomes.
Catalase is vital because it catalyses the breakdown of harmful Hydrogen Peroxide into water and oxygen. Without Catalase, Hydrogen Peroxide accumulates in the liver and effectively dissolves the organ.
This is potentially fatal. Catalase is specific to Hydrogen Peroxide- it will not Catalase any other reaction. This is because of its specific shape. The oxygen formed reacts with the enzymes active site and displaces the water that has formed there from the above reaction.
This cycle continues until there is no Hydrogen Peroxide left.
Hi there! This is my first visit to your blog! We are a team of volunteers and starting a new initiative in a community in the same niche. Your blog provided us beneficial information to work on. How does temperature affect the rate of reaction for Lipase? As the temperature increases, so will the rate of enzyme reaction. However, as the temperature . Each enzyme catalyses only one type of reaction. From basic knowledge, the rate of reaction will affect by the concentration of the reactant. The higher concentration of reactant, the faster the rate of the reaction. In this experiment, hydrogen peroxide acts as substrate and the catalase in potato tissue is .
The method is called the induced fit theory. According to this theory, the substrate fits perfectly into the enzyme active site: How could I measure the rate of reaction? Catalase produces oxygen as it breaks down Hydrogen Peroxide.
This could easily be measured in a quantitative way, as it should vary according to the enzyme effectiveness would put these results into a table, and create a graph. I could use the graph to calculate the gradient,Effects of Temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes, proteins that act as catalysts, are the most important type of protein.
Investigating the Effect of Substrate Concentration on Catalase Reaction - Investigating the Effect of Substrate Concentration on Catalase Reaction Planning -Aim: The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction of the enzyme (Catalase).
Effect of Substrate Concentration on Enzyme Activity Essay - Background An enzyme is a biological catalyst which speeds up biochemical reactions, such as digestion and respiration, but they remained unchanged at the end of the process (Walpole, Merson-Davies, and Dann 53). More Essay Examples on Effect Rubric.
2H2O2 2H2O + O2. However, this process is very lengthy. There is an enzyme known as catalase in cells which dramatically increases the rate of decomposition of hydrogen peroxide. An Investigation into how the Concentration of Substrate will affect the Rate of an Enzyme Controlled Reaction PH The pH of the hydrogen peroxide and of the chemical which is being used to dilute the hydrogen peroxide (water) will affect the rate of the reaction.3/5(1).
Essay about Investigate the effect of enzyme concentration on the rate of reaction - To investigate the effect of enzyme concentration on the rate of reaction PREDICTION: An enzyme is a biological catalyst.